PSI:Biology Policies for Community-Nominated Targets
1. Targets that are nominated by the community should be consistent with the overall mission of the PSI:Biology program. Investigators can request novel protein structures as well as other complexes including proteins of known structure as long as the results of the structural study are novel and of high biological significance.
2. It is important that any targets that are taken on by the PSI:Biology Network have at least the same chance of success as the targets that are already in the overall pipelines of communal targets chosen by the PSI itself. The goal is to provide useful structures to the community with a reasonable success rate so that the PSI can contribute substantially to the community efforts. It should also be noted that the PSI:Biology Network cannot guarantee that proposals will result in a structure.
3. When targets are proposed, a rationale for why these targets should be studied must be outlined in 500 words or less. It is important to provide full sequence information, as well as the sequences for any constructs that have been previously attempted for protein production and/or crystallization. Recommendations as to whether this would be a target for a high-throughput center or a membrane protein structure determination center would also be useful, and whether X-ray or NMR is more suitable. Nominations from foreign investigators are encouraged to consult with the one of the PSI Center Directors prior to nomination.
4. If protein samples are provided by the nominator, then the protocols used for sample production must be well documented and would normally include expression system, purity, stability, buffers and whether the protein can be concentrated to at least 5-10 mg/ml for crystallization studies. Similar sets of feasibility criteria, including demonstration of sample stability and high quality HSQC spectra, would pertain to targets proposed for NMR study.
5. In accordance with PSI Policies, all structure coordinates and structure factors for nominated targets, including any nominations from for-profit organizations, will be deposited within 4 weeks of structure completion and released immediately . All clones leading to successful structures will be deposited in the PSI:Biology-Materials Repository. It is expected that the investigators submitting nominations will make every effort to submit publications based on the PSI work.
approved May 2, 2011